Project title: Development of Biowaste derived Carbon Dots
and Metal Organic Frameworks heterostructures to build a platform for printable
Biosensors
Project Acronym: BMOFSENS
Principal Investigator: Dr.
Disha
Nanoelectrochemistry,
Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw,
Poland,
Email:
disha@ichf.edu.pl
Doctor
of Philosophy
Academy of Scientific and Innovative
Research (AcSIR-CSIO) [18 Aug 2017-01 May 2023]
Address: CSIR-CSIO Sector 30C, 160030 Chandigarh
(India) http://acsir.res.in
Thesis: Development of carbonaceous
nanostructures based biosensing platform for steroid hormone sensing.
Work experience
Junior
Research Fellow under UGC project
Central Scientific Instruments Organization, Chandigarh,
India. [18 Aug 2017 – 17 Aug 2019]
Project: Development of carbonaceous
nanostructures based biosensing platform for steroid hormone sensing.
Research Work: related to the nanostructures and
their composites-based development of electrochemical bio sensing platforms.
Senior Research Fellow under UGC project
Central Scientific Instruments Organization, Chandigarh,
India. [18 Aug 2019 – 30 Jun 2022]
Project: Development of carbonaceous
nanostructures based biosensing platform for steroid hormone sensing.
Research work: related to the synthesis and
characterization of various nanomaterials mainly includes graphene quantum dots
and magnetic nanocomposites of graphene oxide for the development of rapid
immunosensor for steroid hormone. Testing and real applications of the
developed sensor.
Senior Research Fellow under ICMR project
Central Scientific Instruments Organization, Chandigarh,
India. [01 July 2022 – 30 June 2023]
Project: Development of Molecularly imprinted
polymers opto-electrochemical sensors for the detection of steroid hormone
Research work: related to the synthesis and
characterization of molecular imprinted polymers and carbon dots composites for
the development of biosensors.
Mentor: dr inż. Wojciech Nogala
Nanoelectrochemistry,
Institute of Physical Chemistry, Polish Academy of Sciences,
Warsaw,
Poland, +48 22 343 3375.
wnogala@ichf.edu.pl
Source of funding: This
research is part of the project no. 2022/47/P/ST5/02424
co-funded by the National Science Centre and the European Union’s Horizon 2020
research and innovation programme under the Marie Skłodowska-Curie
grant agreement No. 945339.
Project description for the general
public
A
biosensor produces readable signals proportional to the concentration of an
analyte (e.g., biomolecules) in a reaction to identify the biological or
chemical processes. There are several uses for biosensors, including disease
monitoring (e.g., cancer), drug research, detection of contaminants, pathogens
(e.g., food pathogens), and disease (e.g., COVID-19) indicators in
physiological fluids (blood, urine, saliva, sweat). Due to its vast range of
applications related to health and the environment, the design and development
of high-performance biosensors have captured the considerable attention of
researchers and scientists in the last ten years. The biosensor market is
expected to double in the coming 10 (ten) years. Among biosensors, many
biosensing technologies are available, including electrochemical, optical,
plasmonic, etc. However, 80% of the market is dominated by electrochemical
biosensors alone. Among electrochemical biosensors, the efficient capture of
biorecognition signals and their conversion into electrochemical signals
(transduction process), improving transducer performance (i.e., increasing
sensitivity, reducing response time, reproducibility, and lowering the
detection limits even to detect trace level or individual molecules), and
miniaturization of the biosensing devices are the main challenges involved in
the development of biosensors. The performance of the electrochemical
biosensors highly depends on the material platform used to immobilize the
bioreceptors. The advancement of the materials at the nanoscale has allowed the
production of highly accurate electrochemical biosensors. The combination of
sensing technologies with nanomaterials—which can be zero to three dimensional,
have a high surface-to-volume ratio, and strong conductivities, can address the
issues that are being generally faced with respect to the sensitivity,
repeatability, and selectivity. Numerous nanomaterials have been used in
biosensing technologies, out of which carbon dots (CDs) have emerged as an
intriguing contender for immobilizing biomolecules and building biosensors due
to its low cost, strong affinity, surface functionalities, high surface area,
superior intracellular solubility, and nontoxicity. CDs are the result of
limiting carbon material's physical dimension, which can deliver the new
physical and chemical properties usually absent in the bulk counterparts.
Further deriving these carbon dots from the biowaste (BWCDs) is highly
appreciable, targeting the waste to wealth. However, still creating a single
material platform for a wide range of biomolecules is a challenge that demands
the control of material characteristics (surface functionalities, redox
centers, surface area, stability, conductivity, and diffusion properties) at
the nanoscale. To address this issue, producing desirable out-of-plane
functionalities (will enhance the functional group density) and covalent
bridges with highly porous and tunable host structures could be an ideal
platform for biosensing. Coming to the porous materials, recently developed
metal organic frameworks (MOFs) structure allow the myriads of metal clusters
and organic linkers combination to tune the porosity and chemical/physical
properties. In this project we propose the hybrid structures of BWCDs and MOF
as a base platform to immobilize wide range of bioreceptors just by changing
functionalities at the base material (functional groups at BWCDs, metal centers
for MOF, and the spatial distribution of BWCDs in MOF crystals). The developed
material is expected to have easy structural modification for different types of
bioreceptors with high performance. Further, in order to enhance the
performance of the biosensors based on the developed material, a new technique,
wiz scanning electrochemical microscopy, will be explored, which can allow for
the extension of the limit of detection by studying the electrochemical
reactions at a micro-scale dimension, could allow detecting the minute
concentration of target biomolecules which is one of the main challenges in the
biosensor industries.
Project location: Institute of Physical
Chemistry, Polish Academy of Sciences, Warsaw, Poland
Research
group: Nanoelectrochemistry
Group leader:
dr inż. Wojciech
Nogala
The
group deals with fundamental aspects of charge transfer processes using high
resolution electroanalytical methods. Besides others, scanning electrochemical
microscopy (SECM) and scanning ion conductance microscopy (SICM) with
nanoelectrodes and nanopipettes as probes are used. In particular the group is
interested in electrochemical studies of nanostructures, nanoscale mapping of
activity and understanding of biocatalytic processes down to single molecules. https://ichf.edu.pl/en/groups/nanoelectrochemistry.
Contact details: ul. M. Kasprzaka 44/52, 01-224
Warszawa, mazowieckie, Poland
Phone Number:
(22) 343-31-08
E-mail: ichf@ichf.edu.pl
WWW: www.ichf.edu.pl
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